Semi-automated method for the differential determination of plasma catecholamines
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چکیده
منابع مشابه
Semi-automated method for the differential determination of plasma catecholamines.
A method is described for the estimation of adrenaline and noradrenaline in a 5 to 10 ml plasma sample.A batch technique of adsorption and elution from alumina is used and the final estimation is carried out using an automated fluorimetric technique based on the trihydroxyindole method. Fluorescence is stabilized first by thioglycollic acid to give noradrenaline concentrations only and then by ...
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متن کاملAn automated trihydroxyindole procedure for the differential analysis of catecholamines.
An automated trihydroxyindoleprocedure for the differential estimation of epinephrine and norepinephrinein tissues,urine, and blood is presented in detail. After an initial purification of the catecholaminesby adsorption on aluminum oxide, the neutralized eluates are subjected to automatic analysis, in which potassiumferricyanide and an alkaline stabilizing agent are automatically mixed sequent...
متن کاملModified fluorometric method for determining plasma catecholamines.
Plasma catecholamines were fluorometrically determined by a modification of the standard alumina-trihydroxyindole method in which radioactive tracer catecholamines are used to follow the chromatographic behavior of the endogenous catecholamines on alumina. This modification permits appropriate fluorometry of that portion of the eluate with the highest concentration of catecholamines, because in...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1968
ISSN: 0021-9746
DOI: 10.1136/jcp.21.6.759